The firm is based in North Ryde, Australia, and is backed by the Australian National Science Agency, CSIRO, the Australian Federal Government, and private investors. Main Sequence Ventures invests in early and seed-stage companies that focus on humanity-scale healthcare, exponential machines, farming and food, space and transport, learning, and imagining. They leverage world-leading research from Australian public research organizations such as CSIRO and universities to accelerate new companies and industries that tackle the world's biggest challenges. Since its establishment in 2017, Main Sequence Ventures has invested in 39 companies, leading to over 60 company collaborative projects with universities and 19 projects with CSIRO.