Matadero Capital focuses on investing in seed, growth, and late-stage ventures in the life science and healthcare sectors. They also make real estate investments in the life science sector. The firm has a higher than average amount of lead investments and has had successful exits. They have invested in startups in fields such as Machine Learning and Natural Resources, with notable portfolio companies including Valor Water Analytics, InterVenn, and boomtrain. Matadero Capital typically invests in startups with 3 or fewer founders and prefers to invest in companies that are 4-5 years old. They often participate in investment rounds with 4 other investors and have been sponsored by other venture capital firms such as Sierra Ventures, Y Combinator, and Tumml.